日本血吸虫抱雌沟蛋白基因表达的性别与时相差异性研究

SEX-SPECIFIC AND STAGE DIFFERENT EXPRESSION OF GYNECOPHORAL CANAL PROTEIN GENE OF SCHISTOSOMA JAPONICUM(Chinese strain)

用Southernblotting ,Northernblotting技术对所克隆的日本血吸虫抱雌沟蛋白编码基因SjGcp进行了性别和时期差异表达研究。结果显示日本血吸虫抱雌沟蛋白基因为雄虫特异性表达基因 ,在感染后第

Gynecophoral canal protein shown specific expression in the Gynecophoral canal of male worm and transfer to the female worm.The pairing character of male and female worm was commonly detected after infecting on the 14dyas in the final host.In our research,Southern blotting and Northern blotting were employed to detect the sex-specific and stage-different expression of the SjGCP gene of Schistosoma japonicum.The Southern blotting result shown SjGcp was being in the genome not only of female worm but also of male worm.The Northern blotting results displayed that SjGcp was a male-specific expressing gene,which was detected to be transcripted after infecting of final host when it was just pairing between male and female worm on fourteenth day in the male worm.