摘要
目的 :为探讨糖尿病患者易患动脉粥样硬化是否与结缔组织生长因子有关以及可能的干预药物。方法 :建立大鼠糖尿病模型 ,随机分为正常对照组、糖尿病组、糖尿病盐酸氨基胍治疗组和糖尿病葛根素治疗组。应用逆转录聚合酶链式反应检测各组大鼠主动脉结缔组织生长因子mRNA表达水平。结果发现 ,糖尿病组大鼠主动脉结缔组织生长因子mRNA明显高于对照组 (P <0 .0 1) ,葛根素治疗组和氨基胍治疗组结缔组织生长因子mRNA明显低于糖尿病组 (P <0 .0 1) ,氨基胍治疗组、葛根素治疗组与对照组比较有显著差异 (P <0 .0 5 )。结果 :结缔组织生长因子过多表达与糖尿病患者易患动脉粥样硬化有关。结论 :氨基胍。
Objective:To study mechanism for accelerated atherosclerosis in diabetic patients. Connective tissue growth factor (CTGF) mRNA in the aorta of diabetic rats was observed,at the same time,effects of aminoguanidine and puerarin on the expression of connective tissue growth factor were observed too. Methods:Established diabetic rats by streptozotocin were divided into diabetic rats,diabetic rats treated with aminoguanidine,and diabetic rats treated with puerarin at random.Reverse transcription polymerase chain reaction(RT-PCR)was applied to detect the expression of CTGF mRNA in the aorta. Results:The expression of CTGF mRNA in the diabetic aorta was increased(P<0.01), aminoguanidine and puerarin treatment inhibitsCTGF mRNA overexpression in the diabetic aorta(P<0.01). Conclusion: CTGF might involve in the development and acceleration of atherosclerosis in diabetes, aminoguanidine and puerarin treatment inhibited CTGF mRNA overexpression in the diabetic aorta.
