GSTM1和GSTT1基因多态性与COPD易感性的研究

A Study on the Correlation between the Polymorphism of GSTM1 and GSTT1 Genes and Susceptibility to the Development of COPD

目的 探讨GSTM1、GSTT1基因多态性与COPD易感性的关系。方法 应用PCR技术对 91例COPD患者和 44例非COPD人群GSTM1、GSTT1不同基因型进行检测并根据性别、年龄及吸烟史对其易感性进行分析。结果 两组之间相比较男性及 >40岁的人群其GSTM10 / 0基因型频率有显著性差异 (P <0 0 5 ) ;在吸烟指数为≥ 3 1PY时 ,COPD组的GSTM10 / 0基因型频率显著高于对照组 (P <0 0 5 ) ;根据性别、年龄组及吸烟史分析 ,在两组人群中GSTT10 / 0基因型频率均未发现有显著性差异 (P值均 >0 0 5 ) ;两组人群综合基因型频率分析显示 ,在吸烟指数≥ 3 1PY的人群中GSTM10 / 0 -GSTT10 / 0基因型频率则显著高于对照组 (P<0 0 5 )。结论 GSTM1纯合缺失基因型可能增加了长期大量吸烟人群对COPD的易感性 。

Objective To study the correlation between the polymorphism of GSTM1 and GSTT1 genes and the susceptibility to the development of COPD. Methods Polymerase chain reaction (PCR) was used to detect the GSTM1 and GSTT1 genotypes in 91 cases of the patients with COPD (COPD group) and 44 cases of the subjects without COPD (control group), and their susceptibility to the development of COPD were analyzed according to their gender, age and smoking history. Results There was significant difference in the frequency of GSTM1 0/0 genotype between the two groups of male persons with more than 40 years old (P<0 05). The frequency of GSTM1 0/0 genotype in COPD group was higher than that in control group in the individuals with an average smoking history of ≥31 pack-years (P<0 05). The significant differences in the frequency of GSTT1 0/0 genotype were not found between the two groups according to their gender, age and smoking history. The frequency of both GSTM1 null and GSTT1 null genotypes(GSTM1 0/0-GSTT1 0/0) in COPD group with an average smoking history of pack-years≥31 was higher than that in control group(P<0 05). Conclusion The risk for COPD in the subjects of heavy smokers may be mainly affected by GSTM1 0/0 genotype, and possibly be reinforced by the effect of GSTT1 0/0 genotype.