利用AFLP和SSR标记构建美洲黑杨×青杨遗传图谱

Genetic Linkage Mapping in F_2 Population of Populus deltoides×P. cathayana by Using AFLP and SSR Marker

以美洲黑杨×青杨F2代为作图群体,利用AFLP和SSR标记技术构建分子连锁图谱。连锁图共有19个较大的连锁群,16个二联体(Doublets),7个三联体(Triplets)和5个较小的连锁群。19个较大的连锁群上包括356个AFLP标记和12个SSR标记,总图距为3382 4cM,标记间的平均间距为9 69cM。估计基因组长度为2607～3319cM,平均为3042cM,同时采用Lander和Bishop的方法计算期望基因组覆盖度,平均值分别为96 7%和98 4%。

Based on the techniques of amplified fragment length polymorphism (AFLP) and microsatellite (SSR) marker, a hybrid molecular linkage map of Populus deltoides×P. cathayana was constructed. 19 major groups, 16 doublets, 7 triplets and 5 small groups were identified in this map.356 AFLP makers and 12 SSR markers, total 368 makers, were ordered on the 19 major groups. This map covered 3 382.4 cM of genome length, and the average distance between two markers was 9.69 cM. The estimated genome length was between 2 607 cM and 3 319 cM and was 3 042 cM in average. The expected genome coverage was calculated and it was 96.7% with the equation of Lander,and it was 98.4% with method of Bishop et al.