猪源嗜麦芽窄食单胞菌16S rRNA基因的克隆和序列分析

Cloning and sequencing of 16 S rRNA gene of Stenotrophomonas maltophilia from swine

从临床病猪无菌采集抗凝血 ,抽提全血基因组DNA ,用能够扩增大多数真细菌 16SrRNA基因的通用引物 ,扩增出长度为 15 0 2bp的嗜麦芽窄食单胞菌的 16SrRNA基因 ;该基因与国外报道的嗜麦芽窄食单胞菌部分临床和环境分离株核苷酸序列的同源性达 99%以上。证实嗜麦芽窄食单胞菌可感染猪。

Stenotrophomonas maltophilia has been increasingly reported as nosocomial opportunistic pathgen, and responsible for serious infectious complications in immunocompromised patients.At present no information is reported on its infection and pathogenicity in animals.In this study. whole blood genomic DNA was extracted from clinical piglets. A 1 502 base-pair(bp) fragment of the 16 S rRNA gene of Stenotrophomonas maltophilia was amplified using the universal eubacterial primers. The result indicated that Stenotrophomonas maltophilia can infect pigs.