猪胸膜肺炎放线杆菌PCR检测方法的建立

Establishment of PCR method for detection of Actinobacillus pleuropneumoniae

根据猪胸膜肺炎放线杆菌 (APP)外膜脂蛋白基因序列 ,设计合成了 1对特异性引物。经PCR扩增 ,APP 110标准血清型菌株均能扩增出大小为 980bp的DNA片段 ,而大肠埃希氏菌、猪多杀性巴氏杆菌、猪链球菌、猪肺炎霉形体和葡萄球菌等的扩增结果均为阴性。该方法检测APPDNA的敏感性可达 2pg。表明 ,此PCR方法特异性好 ,敏感性高 ,可用于猪传染性胸膜肺炎的快速诊断。

According to nucleic acid sequence of outer membrane lipoprotein gene of Actinobacillus pleuropneumoniae (APP), a pair specific primers were designed. With the primers, DNA fragments 980 bp in length were amplified from 10 serotypes of APP standard strains by PCR. The sensibility of the PCR method is 2 pg. It was concluded from the above-mentioned results that the established PCR method can be used to diagnose quickly porcine infectious pleuropneumoniae.