菌寄生真菌粉红聚端孢s24的Trichothecin(TCN)纯化和抗菌活性

Purification and Antifungal Activity of Trichothecin(TCN) from Mycoparasite Trichothecum roseum s24

Trichothecenes是具有潜在抑制真核生物蛋白质合成功能的毒素,多由丝状真菌代谢产生。利用棉花田间分离到的菌寄生真菌粉红聚端孢菌s24菌株,在其他培养条件一致的前提下,分别采用2种MS培养基(有无几丁质作为碳源)对其进行培养,10 d后收集培养滤液,培养滤液经3-M滤纸过滤后用氯仿:异丙醇(1:1)抽提,分别获得0.20 g和0.13 g粗产品,然后再经Kieselgel 60硅胶柱层析、Reverse—HPLC和制备型TLC展层分离,获得0.11 mg和0.04 mg Trichothecin(TCN)毒素,其中以几丁质为主要碳源所得毒素的相对含量明显大于前者的相对含量。TLC-BIOASSAY抗菌试验证实,该毒素能有效抑制病原真菌的生长。

Trichothecenes are a group of biologically active mycotoxins produced by several fungi and have a cytotoxinc effect on eucaryotic by resisting protein synthesis. In this research, a mycotoxin was extracted and purified from a mycoparasite fungus, Trichothecium roseum s24 isolated from natural cotton field, and showed obvious yield difference when T, roseum s24 was cultured respectively in the two kinds of relatively identical cultural conditions with or without the adding of chitin. The results were that 0.11 mg and 0.04 mg TCN mycotoxins were purified by filtering through filter paper, Kieselgel 60 Silica gel chro-matography, Reverse-HPLC and silica gel thin layer chromatography. It was obvious that chitin leads to yield difference. Antifungal assay by TLC - BIOASSAY showed that the toxin could resist pathogen growt-hand development.