斑节对虾两个种群生化遗传变异的研究

Studies on biochemical genetic variation of two populations of Penaeus monodon Fabricius

采用聚丙烯酰胺梯度凝胶垂直板电泳的方法 ,对斑节对虾 (PenaeusmonodonFabricius)2个种群的苹果酸酶 (ME) ,苹果酸脱氢酶 (MDH) ,谷氨酸脱氢酶 (GDH) ,乳酸脱氢酶 (LDH) ,磷酸葡萄糖变位酶 (PGM) ,碱性磷酸酶 (ALP) ,酸性磷酸酶 (ACP) ,超氧化物歧化酶 (SOD)和酯酶(EST)等9种同工酶做了对比分析。结果表明 ,在2个种群的9种同工酶系统的18个位点中 ,三亚种群 (中国 )有9个为多态位点 ,占位点总数的50.0%;纳颉岛种群 (泰国 )有10个是多态位点 ,占态位点总数的55.6 %。其中PGM -3位点在三亚种群中只出现纯合型 ,在纳颉岛种群中只出现杂合型 ;而MDH -1和ALP-3位点在三亚种群中只出现杂合型 ,在纳颉岛种群中只出现纯合型。三亚种群和纳颉岛种群的平均杂合度分别是0.3596和0.3254 ,两者的遗传距离为0.0620。

Nine isozymes,Malic enzyme(ME),Malate dehydrogenase(MDH),Glucose dehydrogenase(GDH),Lactate dehydrogenase(LDH),Phosphoglucomutase(PGM),Alkaline phosphatase(ALP),Acid phosphatase(ACP),Suˉperoxide dismutase(SOD)and Esterase(EST),of two populations of Penaeus monodon Fabricius were analyzed with graˉdient polyacrytamide gel electrophoresis.Results showed that out of18loci in nine isozymes,9polymorphic loic in the Sanya(China)population(SYP)and10in the Najies(Thailand)population(NIP).Percentage of polymorphic loci were50.0%and55.6%respectively.The loci PGM-3occurred only in isozygote in SYP,in heterozygote in NIP;while the loci MDH-1and ALP-3are heterozygote in SYP and isozygote in NIP.The average heterozygosity was0.3596in SYP and0.3254in NIP respectively;and the genetic distance between the two populations was0.0620.