摘要
本文利用差速离心方法提纯的登革2型病毒、抗登革病毒单抗、病毒受体、耦合有G蛋白的琼脂糖珠之间特异性结合的作用,利用免疫共沉淀方法从C6/36细胞中分离鉴定出分子量约为35kDa受体蛋白;并用糖蛋白染色方法否定了此蛋白的糖基化特征。
In this paper, based on the especial interaction among purificated Dengue 2 virus, monoclon- al antibody against Dengue virus, virus receptor, and Protein-G-Agrose; one non-glyprotein, which molecular weigt is approximately 35kDa, has been identified to be the receptor of Dengue 2 virus on the C6/36 cells by the method of immunological co-sedimentation. The glyprotein nature of this receptor is also denied through glyprotein stain procedure.
出处
《中国病毒学》
CSCD
2004年第3期217-219,共3页
Virologica Sinica
基金
中国人民解放军总后勤部"九五"医学杰出中青年基金课题
