摘要
为观察补骨合剂对骨髓基质细胞 ( MSC)增殖的影响 ,采用贴壁分离筛选法对 MSC进行形态学观察、生长曲线分析 ,用 MTT法检测补骨合剂对 MSC增殖的影响 ,结果发现补骨合剂刺激 MSC增殖与药物浓度有关 ,浓度为 1 0 0 μg/ml时达到最大效应 ,说明补骨合剂可以直接刺激 MSC增殖 ,使成骨细胞转化的数量增加 。
To observe the influence of Bu Gu mixture on the proliferation of marrow stroma cells (MSC),detect morphologic characteristics of MSC,analyze its growth curve by adherent separation screening method,examine the influence of Bu Gu mixture to the proliferation of MSC by MTT method.It was found out that the stimulus of MSC proliferation by Bu Gu mixture is related to the concentration,and the maximal effect will be obtained at the concentration of 100 μg/ml.It was implied that Bu Gu mixture could directly stimulate the proliferation of MSC and increased the transformation of osteoblasts,hence improved the formation of bone.
出处
《福建中医学院学报》
2004年第3期23-26,共4页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建省卫生厅重点课题资助项目 ( No.2 0 0 2 -2 0 1)
关键词
补骨合剂
体外培养
骨髓基质细胞
MSC
中医
Bu Gu mixture
rat
marrow stroma cell
in vitro culture
proliferation
