棉花DNA提取方法的优化及SSR标记在多态筛选的应用

Improved of Total DNA Extraction Approaches in Upland Cotton and Polymorphic Loci Amplified by SSR

对棉花DNA的提取方法进行了优化,通过改良方法所提取的棉花总DNA的质量得以提高。为了进一步证实改良的总DNA提取方法的效果,对所构建的抗黄萎病QTL定位群体的双亲进行了SSR-PCR检测,得到了清晰的多态性SSR标记,为研究分子标记在棉花育种的应用打下了基础。

In this paper,with improved of total DNA Extraction Approaches in upland cotton, total DNA with high quality from the first or second young leaf could be improved. In order to detect the effective of total DNA extraction approaches, the parents ,which were produced QTL map groups for resistant to verticillium wilt trait,is amplified by SSR-PCR. the results showed that ideal amplification bands could be got in SSR -PCR reaction with these DNA. so it can meet the need for the molecular makers utilized in cotton breeding.