摘要
采用无血清培养液分离和培养新生SD 大鼠脑的神经干细胞,以机械分散的方法传代,成功地建立了大鼠脑神经干细胞系(RSNC-FMU 1)。该细胞系可在体外长期传代,至今已在体外连续生长超过21个月(>100代),保持了神经干细胞的特性和正常的核型,经诱导可分化成为神经元、星形胶质细胞和少突胶质细胞,具有较旺盛的自新能力,倍增时间约为20h,并可冷冻保存,裸鼠体内移植证实其不具有成瘤性。该细胞系为神经干细胞研究提供了一个良好的工具。
A neural stem cell line (RNSC-FMU 1) From rat brain have been established suc-cessfully by isolating and culturing neural stem cells from newborn SD rat brain in vitro withfree-serum medium and passaging with mechanical division.The cell line cultured can continuous-ly generale in vitro for long-term and it is 21 months (>100 passages) so far.These cells keepthe feature of neural stem cell and normal karyotype.These neural stem cells can be induce todifferentiate into neurons,astrocytes and oligodendrocytes.The cells have an extensive self-renewalcapacity;its doubling time of proliferation is about 20h.The cells are also cryoprescrvable.Tumorformation is not observed in nude mice that explanted with the cells.This cell line in a good toolfor research of neural stem cell.
出处
《实验生物学报》
CSCD
北大核心
2004年第3期199-204,共6页
Acta Biologiae Experimentalis Sinica
基金
福建省科技计划项目(2001Z020)
关键词
大鼠
神经干细胞系
核型
鉴定
分化
Rat.
Neural stem cell line.
Establishment.
Characterization
