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内蒙古蒙古族人群DYS413基因座的多态性分布 被引量：2

The Polymorphism Distribution of DYS413 locus in Mongolians(Inner Mongolia)
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摘要 为调查DYS413(YCA Ⅲ)基因座在内蒙古蒙古族人群中的分布情况,为其应用于法医学和人类遗传学研究提供依据,采集了120例内蒙古蒙古族男性无关个体静脉血,EDTA抗凝,用酚-氯仿法抽提DNA,PCR扩增DYS413基因座,6％非变性聚丙烯酰胺凝胶电泳,1‰硝酸银(AgNO_3)染色分型。在120例内蒙古蒙古族男性人群中共检出30个不同的单倍型,其频率介于0.0007至0.01361之间,其HD(单倍型多样性)值为0.94054。表明DYS413基因座在内蒙古蒙古族人群中分布好,对法医学和人类遗传学研究具有重要价值。 In order to provide the evidence for the application of DYS413 (YCA Ⅲ) locus in forensic medicine and pop- ulation genetics by investigating its polymorphism in the Mongolians lived in the Inner Mongolia Autonomous Re- gion, China, to collect 120 unrelated male Mongolians' venous blood, who lived in the Inner Mongolia Autonomous Region, and the blood is anticoagulant with EDTA, then to extract DNA by using the method of phenol-chloroform and amplify the DYS413 Iocus with PCR. The products were separated with 6% non-denatured polyacrylamide/bi- sacrylamide gels(19: 1) electrophoresis and stained with 1‰, nitrate of silver(AgNO_3). There are total 30 different haplotypes in Mongolians, the frequencies are from 0. 0007 to 0. 01361, the HD(haplotype diversity) is 0. 94054. It shows that DYS413 locus is high valuable in the application of forensic medicine and population genetics.
作者 王永在 杨国香 王艳 贠克明 郭大玮 王英元
机构地区 山西医科大学法医学院物证教研室 内蒙古医院检验科
出处 《遗传》 CAS CSCD 北大核心 2004年第3期287-290,共4页 Hereditas（Beijing)
基金 山西省归国人员基金(No.9702)~~
关键词 DYS413(YCA Ⅲ)基因座 STR 单倍型 法医学 人类遗传学 DYS413(YCA Ⅲ)locus STR haplotype Forensic medicine Population genetics
分类号 Q987 [生物学—遗传学]
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参考文献10

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