摘要
利用PCR技术和复合电泳银染技术检测奶牛和肉牛BM2113、BM1862、BMc701、BM2934、TGLA122、BM720等6个STR基因座的多态性分布,并计算该6个基因座的基因频率(P_i)、个体鉴别力(DP)、杂合度(H)、多态信息含量(PIC)、和非父排除概率(PE)。结果显示:6个STR基因座的基因型分布符合Hardy-Weinberg平衡,奶牛中6个STR基因座中BM2113基因座的DP、H和PIC最高,TGLA122基因座的PE最高。6个STR基因座的累积个体鉴别力(CDP)为0.99997,累积非父排除能力(CPE)为0.98827。肉牛中6个STR基因座中BM1862基因座的DP、H、PIC、PE都是最高,6个STR基因座的累积个体鉴别力(CDP)为0.99999,累积非父排除能力(CPE)为0.99578。结果表明,6个STR基因座可用于牛的遗传连锁分析、个体识别和亲子鉴定等研究领域。
The polymorphism distributions of six STR loci, BM2113, BM1862, BMc701, BM2934, TGLA122, and
BM720 were detected in cattle by Polymerase Chain Reaction and multiplex gel electrophoresis followed by silver
staining. Gene frequency (P_i ), power of discrimination (DP), heterozygosity (H), polymorphism information con-
tent (PIC) and probability of paternity exclusion (PE) were calculated. All loci obey Hardy-Weinberg equilibrium.
DP, H and PIC of BM2113 locus, and PE of TGLA122 locus are the biggest among six STR loci in Holstein Frie-
sian. Cumulate DP of six STR loci is 0. 99997, Cumulate PE of six STR loci is 0. 98827. DP, H, PIC and PE of
BM1862 loci are the biggest among six STR loci in beef. Cumulate DP of six STR loci is 0.99999, Cumulate PE of
six STR loci is 0.99578. These results showed that the six STR loci could be used as linkage analysis, individual i-
dentification and paternity test in cattle.
出处
《遗传》
CAS
CSCD
北大核心
2004年第3期309-314,共6页
Hereditas（Beijing)
基金
国家自然科学基金(30070555)资助~~
