转基因山羊乳中EPO活性检测

Detection of Biological Activity of EPO Expressed in Transgenetic Goat Milk

目的 对转基因山羊乳中 EPO的生物学活性进行检测。方法 用网织红细胞法检测体内生物活性,用ELISA法和 MTT法检测体外生物活性。结果 羊乳中 EPO体内生物学活性明显低于其体外生物学活性。结论推测我们所得的重组EPO的N糖基高分支的比例小及唾液酸化程度偏低,造成其体内生物活性下降。

Objective To detect biological activity of rhEPO expressed in transgenetic goat milk.Methods The biological activity in vivo of rhEPO was determined by reticulacyte counting method.The biological activity in vitro of rhEPO was determined by ELISA and MTT colorimetric method,respectively.Results The biological activity in vivo of rhEPO was obviously lower than its biological activity in vitro.Conclusion The difference is possibly caused by the glycan structure of rhEPO.