摘要
为研究胡黄连提取物对四氯化碳 (CCl4 )或半乳糖胺 (D GaIN)损伤原代培养大鼠肝实质细胞的保护作用。半原位酶分离法分离大鼠肝细胞 ,利用CCl4 或D GaIN引起原代培养大鼠肝实质细胞损伤模型 ,以培养液中谷丙转氨酶(ALT)活性变化为指标 ,并采用MTT法对细胞活性和增殖进行检测。结果 :胡黄连提取物能够增强受损肝细胞的活性和增殖能力 ,但不能显著降低CCl4 或D GaIN肝损伤模型ALT活性。
The protective effect of the extracts of Rhizoma Picrorhizae on primary cultured hepatocytes injured by CCl 4 and D-GaIN was studied.The hepatocytes were isolated and damaged by CCl 4 or D-GaIN;the activity of ALT,and the activity and proliferation of hepatocytes were measured.Results:The extracts could increase the activity and proliferation of injured hepatocytes,but could not obviously reduce the activity of ALT.
出处
《上海中医药大学学报》
CAS
2004年第2期42-43,共2页
Academic Journal of Shanghai University of Traditional Chinese Medicine
