摘要
对光谱探针天青A(AA)和亚甲蓝 (MB)分别与硫酸软骨素 (CS)、透明质酸 (HA)和硫酸化茯苓多糖 (SP)酸性多糖相互作用的吸收光谱进行了比较研究 ,并分析它们与上述三种酸性多糖的结合反应的特点 ,还测得AA和MB与CS、HA、SP的最大结合数分别为 1 50、3.37× 1 0 3、6 2和 73、54.研究结果表明 ,AA与酸性多糖的磺酸基和羧基均能发生结合反应 ,而MB则只与磺酸基发生结合反应 。
The absorption spectra of the interaction between two spectroscopic probes (Azur A (AA), Methylene Blue (MB)) and three acidic polysaccharides (Hyaluronic Acid (HA), Chondroitin Sulfate (CS) and Sulfated Pachyman(SP)) were comparatively studied. The characters of the interactions between the two probes and three acidic polysaccharides were compared. The maximum binding numbers of the two probes with three acidic polysaccharides have been obtained, to be 150, 3.37×103, 62 and 73, 54, respectirely. The results showed that AA could interact with both the sulfate group and the carboxylic group of the acidic polysaccharides while MB could only interact with the sulfate group.
基金
国家科技攻关项目 (No .2 0 0 2EB0 2 0 96 2 )
关键词
天青A
亚甲蓝
酸性多糖
吸收光谱
Azur A
Methylene Blue
acidic polysaccharides
absorption spectra
