大白菜无菌苗子叶组织培养再生植株

Plant Regeneration from Cotyledons of Chinese Cabbage Cultured in vitro

选用8个大白菜亲本材料进行离体培养试验,探讨了植物激素、品种、苗龄、取材部位及外植体放置方式对大白菜子叶不定芽再生的影响。研究结果表明,大白菜子叶再生培养基为MS附加6-BA 2 mg/L,NAA 0.5 mg/L和AgNO3 5 mg/L;生根培养基为1/2MS附加NAA 0.2 mg/L。再生植株移栽时,最好在春季,成活率可达90％以上。

Eight parental lines of Chinese cabbage was cultured in vitro. Effect of hormone combinations, different genotypes, ages of seedlings etc on shoot and plant regeneration from cotyledonary explants of Chinese cabbage was discussed. Maximum shoot regeneration was obtained in MS medium complementing with 2 mg/L 6-BA.0.5 mg/L NAA and 5 mg/L AgNO3.Medium for root was MS containing 0.2 mg/L NAA. The survial rate of transplanting plantlets was as high as 90 % in Spring.