胡麻枯萎病病原尖孢镰刀菌生态生物型的划分研究

Studies on Ecotype of Flax Wilt-inducing Pathogenic Organisium(Fusarium oxysporum f.sp.lini)

通过酯酶同工酶技术将来自中国主要胡麻产区内蒙古、山西、河北、甘肃的17个枯萎病菌株划分4个酯酶型,同时对部分菌株采用人工室内接种同一胡麻材料后的病情进行分析,考察菌株间的致病性。结果表明:同一地区的菌株属于同一酯酶型,同一地区的菌株间的致病性差异不显著,不同地区菌株间的致病性差异显著。

17 wilt-inducing isolates of Fusarium oxysporum f.sp. lini were obtained from the diseased flaxs from the four main flax pruduction regions, Inner Mongolia, Shanxi province, Hebei province, Gansu province, through cellection, isolation, single macroconidia purification, these isolates were classified into four types of es-terase isoenzyme by the electrophoretic technology, and the isolates from the same region belonged to the same type of esterase isoenzyme,so we called the types of esterase isoenzyme the ecotypes. The high susceptible flax line, 7528-4-2-1,was respectively inoculated by eight isolates. Result showed that the wilt-inducing abilities of the isolates coming the same region are not significant,that from different regions are significant.