应用显微分光光度计检测大肠癌及大肠其它病——DNA含量与发病学关系的研究

DETERMINATION OF DNA CONTENTS IN COLORECTAL CARCINOMA AND OTHER COLORECTAL DISEASES WITH MICROSPECTR OPHOTOMETR YAND STUDY ON THEIR ETIOLOGICAL RELATIONSHIP

应用显微分光光度计分别对大肠癌、癌旁组织、慢性结肠炎、腺瘤性息肉和几种非腺瘤性息肉标本进行DNA定量分析。结果显示所有大肠癌标本DNA含量明显高于正常粘膜、慢性结肠炎、腺瘤(伴轻、中度不典型增生)和几种非腺瘤性息肉。DNA含量在腺瘤中随不典型增生程度的加重而明显增加,支持关于“腺瘤——癌演续”的理论,且表明这一演续过程为多步骤性。而其它几种非腺瘤性息肉则未发现与大肠癌之间有明显发病学关系。

Tissue specimens of colorectal carcinoma, mucosa adjacent to carcinoma, chronic colitis, adenoma, nan-adenomatous polypses were examined with the method of DNA quantitative analysis using microspectrophotometry: The sresults showed that the DNA contents of all specimens of colorectal carcinoma were significantly higher than those of normal colorectal mucosa, chronic colitis, adenomas (with mild, moderate dysp-lasia) and non-adenomatous polypses. The results that DNA contents increased in adenoma along with the degree of dysplasia gived a support to the theory of 'adenoma-carcinoma sequence' and suggested that this sequencial procedure was of multiple-stage. No significant relationship between colorectal carcinoma and non-adenomatous polypses was found.