细胞内钙离子螯合剂抑制HSV-1诱导Hela细胞的凋亡作用

Inhibition of intracellular Ca^(2+) -chelating agent on Hela cell apoptosis induced by HSV-1

目的 :探讨HSV 1在诱导Hela细胞凋亡中 ,细胞内游离钙浓度的变化及钙离子螯合剂对HSV 1诱导Hela细胞凋亡的抑制作用。方法 :HSV 1感染Hela细胞后 ,用扫描电镜及透射电镜观察凋亡情况。用荧光探针标记细胞内游离Ca2 + ,在不同时间观察细胞内游离Ca2 + 浓度的变化。结果 :HSV 1感染Hela细胞后 ,出现了典型的凋亡形态学变化。细胞内游离Ca2 + 浓度在HSV 1感染Hela细胞后 12h达高峰 ;典型的凋亡形态学变化出现在HSV 1感染Hela细胞后 2 4h。细胞内Ca2 +螯合剂能显著抑制HSV 1诱导的Hela细胞凋亡。结论 :细胞内游离Ca2 + 在HSV 1诱导的Hela细胞凋亡过程中 ,具有重要作用 ,此实验结果为临床治疗相关疾病提供了有用的线索。

AIM: To explore the variation of intracellular free Ca 2+ concentration during Hela cell apoptosis induced by HSV 1 and inhibition of Ca 2+ chelating agent on the apoptosis. METHODS: Apoptosis of the Hela cells infected by HSV 1 was observed under scanning electron microscope (SEM) and transmission electron microscope (TEM), and the variation of intracellular free Ca 2+ concentration, labeled with fluorescent probe was observed under Fluorescence microscope at a series of timepoints. RESULTS: The Hela cells infected by HSV 1 showed typical apoptotic morphological changes. Free Ca 2+ concentration increased and reached peak at the 12 hours after HSV 1 infection. Characteristic morphological features of apoptosis occured at the 24 hours after infection. This apoptosis can be inhibited by intracellular Ca 2+ chelating agent. CONCLUSION: Intracellular free Ca 2+ might play an important role in Hela cell apoptosis induced by HSV 1, which would provide useful clues for clinical treatment of associated diseases.