淋巴细胞中NF-κB p65活性水平在Fx1A诱导的口服耐受大鼠中的变化

Change of activity of NF-κB p65 in lymphocytes from rat with oral tolerance induced by Fx1A

目的 :探讨核因子 κB(NF κB)p6 5在口服耐受发生中的活性改变及其意义。方法 :雌性Wistar大鼠 30只随机分成两组 :①口服耐受组 ,用小剂量 (每次 8mg/2mLPBS)的Fx1A抗原灌胃。②对照组 ,用PBS 2mL/次灌胃。观察大鼠迟发型超敏反应 (DTH) ,进行脾淋巴细胞增殖试验 ,了解大鼠免疫功能的改变。以免疫组化和ELISA法 ,检测大鼠淋巴组织中NF κBp6 5的活性及TGF β1的表达。结果 :与对照组相比较 ,口服耐受组大鼠DTH和抗原特异性淋巴细胞增殖反应明显受抑制 ;肠黏膜Peyer’s淋巴结 (PP结 )中NF κBp6 5的活性及TGF β1的表达明显增加。脾淋巴组织中NF κBp6 5的活性降低 ,但TGF β1的表达明显增加。 结论 :低剂量Fx1A抗原诱导的口服耐受的发生 ,可能与不同部位免疫细胞内NF κBp6 5的活性改变有关。

AIM: To explore the activity change and significance of NF κB p65 in lymphocytes from rat with oral tolerance induced by Fx1A. METHODS: 30 female Wistar rats were divided into two groups (oral tolerance and control groups), which stomachs were perfused with Fx1A and PBS, respectively. The delayed type hypersensitivity (DTH) was evaluated by skin test, the immune function was detected by splenic lymphocyte proliferation test, the activity of NF κB p65 and expression of TGF β1 in lymphoid tissues were examined by immunohistochemical staining and Sandwich ELISA. RESULTS: In comparison with control group, the DTH and antigen specific lymphocyte proliferation reaction in oral tolerance group were suppressed significantly; the activity of NF κB p65 and expression of TGF β1 in Peyer’s patch were increased notably; however, the activity of NF κB p65 was decreased, but expression of TGF β1 was increased markedly in splenic lymphocytes. CONCLUSION: Information of oral tolerance induced by Fx1A may be related to the change of NF κB p65 activity in lymphocytes of body’s different places.