乙酰肝素酶、层黏连蛋白及其受体的表达以及在卵巢癌转移中的作用

The Expression role of acetyl-heparanase, laminin and laminin receptor and their role in the metastasis of ovarian cancer

目的 :探讨乙酰肝素酶mRNA(acetyl heparanasemRNA)、层黏连蛋白 (laminin ,LN)及其受体 (lamininrecepter,LR) ,在 5 0例卵巢癌、33例淋巴结转移卵巢癌及 10例浆液性囊腺瘤中的表达 ,及其在卵巢癌转移中的作用。方法 :应用原位杂交和免疫组化染色方法 ,检测乙酰肝素酶mRNA转录水平及LN和LR的蛋白的表达及定位。结果 :乙酰肝素酶mRNA在卵巢癌病灶及转移淋巴结中的转录水平明显增强 ,原发灶与转移灶之间的表达差异显著 (P <0 .0 5 ) ,恶性肿瘤与良性肿瘤之间表达的差异更为明显 (P <0 .0 1)。LN在癌组织及转移淋巴结中的表达少 ;而LR的表达却明显增强。乙酰肝素酶mRNA与LN表达呈负相关 ,与LR的表达呈正相关。结论 :乙酰肝素酶mRNA的表达与LN和LR的表达之间分别呈正、负相关性 ,提示乙酰肝素酶是参与肿瘤的生长。

AIM: To explore the expressions of acetyl heparanase mRNA, laminin (LN) and laminin receptor (LR) in 50 ovarian carcinoma, 33 ovarian carcinoma with lymph node metastasis, and 10 serous ovarian cystadenoma as well as their role in the metastasis of ovarian cancer. METHODS: The transcription level of acetyl heparanase mRNA, expressions of LN and LR were detected by in situ hibridization and immumnohistochemical staining, respectively. RESULTS: The transcription level of acetyl heparanase mRNA in ovarian carcinoma tissue and metastatic lymph nodes increased significantly, but its expression in primany focus was natably higher than that in metastatic lymph nodes ( P <0.05). There was low expression of acetyl heparanase mRNA in serous ovarian cystadenoma. The expressionof acetyl heparanase mRNA in malignant and benign tumer tissues had markedly differmence ( P <0.01). Expressions LN in both tissues mentioned above decressed while LR expression was high. The expression of acetyl heparanase mRNA was negative correlation with that of LN, while positive with that of LR. CONCLUSION: The correlation among expressions of acetyl heparanase mRNA, LN and LR suggests that heparanase is involved in the growth, invasion and metastasis of ovarian carcinoma.