摘要
目的 :观察不同浓度的胶质细胞源性神经营养因子 (GDNF) ,对大鼠胚胎脊髓运动神经元生长活性的作用。方法 :取大鼠胚胎脊髓腹侧组织体外分离 ,进行原代细胞培养 ,应用抗神经微丝单克隆抗体 (mAb)SMI32进行运动神经元的免疫细胞化学染色 ,从细胞形态学及应用MTT比色法 ,研究GDNF对大鼠脊髓运动神经元的影响。结果 :GDNF能明显促进体外培养的大鼠脊髓运动神经元存活及突起的生长 (P <0 .0 5 ) ,且具有剂量依赖的趋势。结论 :不同浓度的GDNF对体外培养的大鼠胚胎脊髓运动神经元 。
AIM: To observe the effects of various concentrations of glia cell line derived neurotrophic factor (GDNF) on the growth of cultured spinal motor neurons from embryonic rat. METHODS: Neurons were isolated from embryonic rat spinal ventral tissue and then were primarily cultured in vitro . Morphological feature of neurons was examined by immunocytochemical staining with mAb SMI 32. The effect of GDNF on growth of spinal motor neurons was detected by MTT colorimetry. RESULTS: GDNF could significantly enhance the survival and neurite outgrowth of rat spinal motor neurons cultured in vitro in dose dependent tendency. CONCLUSION: Varying concentrations of GDNF can enhance the growth of embryonic rat spinal motor neurons to various extent.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第5期490-492,共3页
Chinese Journal of Cellular and Molecular Immunology