腺苷和抗IgE抗体对哮喘患者BALF中肥大细胞分泌组胺的影响

The Effects of adenosine and anti-IgE antibody on histamine release from mast cells in BALF of asthma patients

目的 :研究支气管哮喘 (哮喘 )患者支气管肺泡灌洗液 (BALF)中肥大细胞的功能特性。方法 :2 9例轻度哮喘患者BALF中的细胞经冲洗后 ,加入含抗IgE抗体、腺苷、缓冲液或腺苷 +抗IgE抗体的LP4试管中进行激发试验。检测BALF肥大细胞中的组胺释放量。结果 :腺苷浓度达 10 0 μmol/L时 ,仍无明显刺激组胺释放的作用 ,仅在浓度高达 10 0 0 μmol/L时 ,方可诱导哮喘患者BALF中肥大细胞释放约 17%的组胺。抗IgE抗体的浓度大于 1× 10 -4g/L时对哮喘患者BALF中肥大细胞的释放组胺有明显的促进作用 ;仅 3× 10 -5g/L的抗IgE抗体与腺苷联合应用的实测值 ,明显高于对应的单独作用组的相加值。结论 :哮喘患者BALF中的肥大细胞对腺苷的刺激反应较差 ,但对抗IgE抗体刺激的反应性明显增强。仅低浓度的抗IgE抗体和腺苷具有协同作用。

AIM: To investigate the functional properties of mast cells in bronchoalveolar lavage fluid (BALF) of asthma patients. METHODS: 29 asthma patients in the recovery phase were enrolled in the study and their bronchoalveolar lavages(BAL) were inspected under fibreoptics bronchoscope. The cells from each BAL fluid(BALF) were added to LP4 tubes containing anti IgE antibody, adenosine, buffer or anti IgE antibody+ adenosine, and incubated at 37℃ for 20 min. The reactions were stopped by placing the tubes on ice and adding 100 μL of cold incomplete Hank’s balanced salt solution (HBSS). The histamine chromogenic assay was performed to detect histamine released from mast cells in BALF. Histamine release was expressed in a net release percentage. RESULTS: 100 μmol/L of adenosine failed to induce histamine release from mast cells; while 1 000 μmol/L of adenosine could induce about 17% histamine release from mast cells in BALF; For anti IgE antibody, as low as 1×10 -4 g/L of anti IgE was able to induce approximately 20% histamine release from the mast cells. When the anti IgE antibody was combined with adenosine, only 3×10 -5 g/L of anti IgE antibody+ adenosine could exert synergistic effectiveness. CONCLUSION: Mast cells in BALF from asthma patients had a poor reaction to adenosine stimulation, but are much more sensitive to anti IgE antibody dependent stimulation; only as low as 3×10 -5 g/L of anti IgE antibody is able to cooperate with adenosine.