乳腺癌激素受体测定——直接荧光法与DCC法比较

COMPARISON OF HISTOCHEMICAL AND DCC ASSAY FOR HORMONE RECEPTORS IN BREAST CANCER

本文对52例乳腺癌标本中ER和PR进行了直接荧光法(FHC法)及葡聚糖包埋活性碳吸附法(DCC法)的配对分析,二种方法在检测ER和PR以及预测内分泌疗效方面有较好的一致性,结果无统计学差异(P>0.5)。对二种方法结果不一致的原因进行了客观分析。由于直接荧光法可进行受体的细胞定位,可检测胞浆、胞核和胞膜受体,不受癌细胞构成比例的影响,且具有操作简便、设备简单、费用较少的优越性,是一种值得推广运用的方法。

Estrogen receptor (ER) in breast cancer tissue from 52 patients and progesterone receptor (PR) from 23 patients were determined by a fluorescent histochemical method (FHC) with 17 -estradiol-6-CMO-BSA-FITC conjugate and Destran-Coated charcoal adsorption (DCC) assay. The result showed that the FHC assay had correlation with DCC assay in determiniing ER and PR The concordance about ER and PR between the two methods were 70% and 78%, respectively. The reason of disagreement between the two methods was discussed. Both methods had similar accuracy to predict the effect of endocrine therapy in 32 patients with breast cancer. The FEC method could located the position of receptors and did not be affected by cellularity of cancer. It has more simple methodology and lower cost, so it might be considered to be a good method for determining hormone receptors.