鼻咽癌细胞EB病毒早期抗原(EA-R)的表达——采用单克隆抗体BAE-5的观察

A PRELIMINARY STUDY ON THE EXPRESSION OF EBV-EA(R) IN NASOPHARYNGEAL CARCINOMA CELLS USING A NEW MONOCLONAL ANTIBODY BAE-5

以正丁酸和巴豆油诱导的Raji细胞为免疫原,应用杂交瘤技术制备一种抗EB病毒(EBV)早期R型抗原(EA-R)的鼠IgM单克隆抗体,命名为BAE-5。以BAE-5为一抗应用APAAP免疫组织化学技术检测34例鼻咽癌和29例非鼻咽癌,结果表明,所有的鼻咽癌(34例)和29例非鼻咽癌肿瘤中的9例能与BAE-5显阳性反应。与已进行EB病毒DNA原位杂交的34例鼻咽癌结果对比认为:大多数鼻咽癌细胞不仅可检测出EBV-DNA,而且这些细胞中的一部分还可表达EA-R。说明这些细胞中的EBV-DNA处于激活状态。一些非鼻咽癌肿瘤能与BAE-5起阳性反应,可能是由于这些肿瘤细胞中也有EB病毒早期抗原存在或者可能存在与EA-R抗原决定簇相类似结构的多肽而引起交叉反应。作者认为这些结果对了解EB病毒有关的多肽,特别是EA复合物在鼻咽癌和一些含EBV-DNA的非鼻咽癌肿瘤的发生和发展中的作用具有理论意义。

The authors prepared a mouse monodonal antibody against EB virus EA-R, named BAE-5, applying hybrktoma technique by use of n-butyric acid and croton oil induced Raji cells as immunogen. And then, 34 NPCs and 29 non-NPC neoplasms were detected by the BAE- 5 usng APAAP immune his tochemical staining method. The results indicated that all of the 34 NPCs and 9 out of the 29 non-NPC neoplasms could react with the BAE-5, in spite of the nasopharyngeal columnar epithelium reserve cells showed positivity also. It is reasonable to come to the conclusion that most of the NPC cells can not only harbour EBV DNA but some of them be able to express EA-R. Some non-NPC neoplasms being demonstrated BAE-5 positivity may be due to the presence of EBV EA-R antigenic epitope or polypeptides simulating the structure of EA-R. The authors Considered that these findings have theoretical significance for understanding the role of EBV-encoded pofypeptides, especially EA complex, in the development and progression of NPC as well as some non-NPC neoplasms harbouring EBV-DNA.