胎盘型谷胱甘肽S—转移酶在肺癌诊断中的应用

APPLICATION OF THE GLUTATHIONE S-TRANSFERASE PLACENTAL FORM IN THE DIAGNOSIS OF HUMAN LUNG CARCINOMAS

应用抗胎盘型谷胱甘肽S—转移酶(GST—π)单克隆抗体,PAP免疫组化染色,对103例原发性肺癌(包括鳞癌31例,腺癌33例,腺鳞癌6例,大细胞癌4例,小细胞癌14例,类癌11例,肺泡细胞癌3例及透明细胞癌1例)进行了检测。结果发现除类癌和小细胞癌外,GST—π在肺癌阳性率高达89.2％(68/78),18例肺良性病变和10例正常肺中,仅1例胎肺呈阳性表达,其余皆阴性。可见GST—π的免疫组化检测可作为肺癌诊断,鉴别诊断的一项新指标。

Human placental form of glutathione S-transferase (GST-π) was detected in the specimens of 103 cases of human primary lung carcinomas, including 31 squa-mous cell carcinomas. 33 adenocarcinomas, 6 adenosquaamous carcinomas, 4 large cell carcinomas, 14 small cell carcinomas, 11 carcinoid tumours , 3 alveolar cell carcinomas, and 1 clear cell carcinoma, by peroxidase anti-peroxidase (PAP) method using monoclonal antibody against GST-π . It was found that GST-π was highly positive at the rates of 89.2% (68/78) in lung carcinomas except carcinoid tumours and small cell carcinomas. Of 18 benign lung lesions and 10 normal lung tissues, only 1 fetal lung was positive, the others were all negative. These results indicated that GST-π is a hovel sensitive marker for immunohistochemical diagnosis and differential diagnosis of lung carcinomas.