测定不同类型肠化生胃粘膜cAMP、DNA和微量元素探索癌变倾向

DETERMINATION OF cAMP, DNA, Zn AND Cu IN VARIOUS TYPES OF GASTRIC MUCOSUA WITH INTESTINAL METAPLASIA SHOWING A CANCERIZATION TENDENCY OF COLONIC METAPLASIA

作者随机抽样观察50例手术切下胃标本粘膜,通过组织化学、放射免疫、自动图象分析仪和能量色散X线分析仪检测,发现①胃粘膜cAMP、Zn、Cu、ZnO与CuO含量,随正常组织至完全性不完全性、小肠型至结肠型、至癌组织的顺序递减。而DNA则随此顺序递增(P<0.05～0.001);②这些物质在不完全性绪肠型肠化生与癌组织间量变无显著性差异,预示前者有癌变倾向。

On the basis of 50 operated gastric specimens, gastric muccsa was continuously sectioned and mucosal tissue imprints were prepared. These slides were studied under the staining of HE, ABpH2.5/PAS, HiD/ABpH2.5, HiD/ABpH2.5/PAS and gastric rnucosa were thus clasffied as normal, complete or incomplete samil intestinal metaplasia -(SIM), complete or incomplete colonic intestinal metaplasia(CIM), and gastric cancerous tissue.These sibes were then subjected to determine trace elements in Wt% and their oxides in Wt% by using type 9100/60 energy chromatic dispersing X-ray analysis instrument. Besides, cAMP levels (pmole/20mg gastric mucosa). were also determined by radio-immuno assay (RIA) technique ; and also the reative levels of DNA in cell nuclei expressed as integral optical optical density (IOD) were determined by using, type IBAS 2000 automatic image analysing system through a study of more than 100 eels on the imprint with Feul-gen stain.Conclusions are:1. cAMP, Zn , Cu, ZnO and CuO were Bdecreased in the sequence from normal to incomplate and complete small intestinal metaplasia, to incomplete and complete colonic metaplasia, and to gastric cancerous tissue.2. DNA were increased in reverse order of above tissue classification.3. The contents of these bio-active substances gave no significant difference between incomplete colonic metaplasia and gastric cancer tissue, probably indicating the transformation from precanceronus to cancerous tissue.