鼻咽癌病人血清中EBV特异性胸苷激酶抗体测定的初步研究

PRELIMINARY STUDIES OF DETECTION OF ANTIBODIES AGAINST THYMIDINE KINASE IN PATIENTS WITH NPC

巴豆油和正丁酸钠诱导B95—8细胞株生成胸腺嘧啶核苷酸激酶(TK),其粗酶液经DEAE纤维素柱层析,可分成两个性质不同的TK活性峰——峰Ⅰ和峰Ⅱ:峰Ⅰ是穿过峰,峰Ⅱ是洗脱峰,洗脱液浓度为172mMk_2 HPO_4。峰Ⅰ酶活性为TTP强烈的抑制作用,不能利用GTP,不被鼻咽癌病人血清所中和。但峰Ⅱ对GTP的利用率高,能被鼻咽癌病人血清所中和,TTP对峰Ⅱ的抑制作用较弱。提示:峰Ⅱ部分是EBV特异性TK。 我们首次用峰ⅡTK来测定NPC(124例)、正常人(56例)、鼻咽部炎症病人(24例),其他头颈部肿瘤病人(26例)血清的TK抗体水平。其阳性率分别为75％、10.71％、8.33％、7.69％,(抗酶率≥30％为阳性)。NPC病人与其他对照组比较,具有显著性差异。实验结果表明:检测EBV——TK抗体可望发展成为NPC诊断的辅助方法而且与现常用EBV血清学检测方法具有互补作用。

J B 95- 8 cells have been effectively induced by a combination of chemical treatment to produce a novel thymidine kinase(TK). After chromatography in DEAE-cellulose Column, the obtained fractions of cellular extracts were tested for TK activity. Two peaks of TK activities were shown. Peak I was eluted in the column void volume and Peak II was eluted at 172 mM K2HPO4 buffer. The biochemical characteristics of two peaks of TK were different and peak II TK was recognized as an EBV-specific TK. We have firstly used the EBV-specific TK in the detection of the neutralizing, antibodies in sera from patients with nasopharyngeal cancer pharyngitis healthy individuals were used as the control. The positive rate was 75%, 7.69%, 8. 33% and 10.71%, respectively AER≥ 30% was designed as positive of TK Ab). Our results suggested that the detection of the level of antibodies angainst EBV-TK may be used in early diagnosis of NPC and is complementary to the test of EDAb, EA/IgA and VCA/IgA, which have been used as routine serological tests in early detection of NPC.