慢性粒细胞白血病慢性期与急变期基因表达差异的研究

Study of gene expression profile of chronic myeloid leukemia in chronic and blastic phases

目的 研究慢性粒细胞白血病 (CML)不同临床阶段的基因表达差异 ,为阐明CML演变的可能机制提供依据。方法 应用DNA芯片技术 ,对CML慢性期与急变期骨髓单个核细胞的基因表达差异进行了检测。结果 在检测的 1176个基因中 ,有 6 8个基因显示急变期较慢性期明显上调 ,其中转录因子 16个 (2 3.5 % ) ,细胞表面抗原 15个 (2 2 .1% ) ,细胞调节蛋白 13个 (19.1% ) ,其它 2 4个(35 3% )。在差异表达的基因中 ,有 17个基因仅在急变期表达 ,而在慢性期不表达 ,11个 (16 .2 % )与G蛋白基因有关。结论 CML慢性期与急变期骨髓单个核细胞基因表达谱存在显著差异 ,基因高表达是CML急变期的特征之一。G蛋白相关基因在CML急变过程中可能起着重要作用。

Objective To study the gene expression profile of chronic myeloid leukemia(CML) in chronic and blast phases for providing information about the potential mechanisms of blastic crisis. Methods The gene expression difference of bone marrow mononuclear cells between CML in chronic and in blastic phases was examined with DNA array. Results In the blastic phase,68 genes were obviously up-regulated in the 1176 tested genes. Among the 68 genes,the transcription factors accounted for 23.5%,cell surface antigens 22.1%,regulation proteins 19.1% and others 35.3%. There were 17 genes expressed only in the blastic phase and 11(16.2%) genes related to G protein. Conclusions Obvious difference of gene expression profile existed between chronic and blastic phases of CML. Up-regulation of gene expression is one of the characteristics of CML in blastic phase. The genes related to G protein may play an important role in the blastic transformation of CML.