易瑞莎对H22肝癌小鼠的抑癌作用

THE EFFECT OF IRESSA ON H22 MOUSE HEPATOCELLULAR CARCINOMA

目的 探讨易瑞莎 (IRESSA)对小鼠H2 2肝癌移植瘤生长的抑制作用。方法 H2 2肝癌移植瘤小鼠随机分为口服对照组、生理盐水对照组、顺铂d1 5组、顺铂d6 10组、易瑞莎组、易瑞莎 +顺铂先合用组、易瑞莎 +顺铂后合用组。易瑞莎用量为 10 0mg/kg体重 ,每日灌胃 1次 ,共 10d(d1 10 )。顺铂用量为 1 2mg/kg体重 ,每日腹腔内注射 1次 ,共 5d(d1 5或d6 10 )。检测肿瘤生长抑制率 (抑瘤率 ,IR)、小鼠体重变化、脾脏指数 (SI)、血白细胞数和血红蛋白值。结果 易瑞莎组抑瘤率与顺铂d1 5组、顺铂d6 10组、易瑞莎 +顺铂先合用组抑瘤率相近 (P >0 0 5 ) ,分别为 4 1%、5 4 %、4 6 %和 5 6 %。易瑞莎 +顺铂后合用组的抑瘤率 (2 6 % )明显低于易瑞莎 +顺铂先合用组 (P <0 0 1)和顺铂d6 10组 (P <0 0 5 )。与口服对照或生理盐水对照组比较 ,易瑞莎组脾脏指数和净体重差异无显著意义 (P >0 0 5 )。易瑞莎 +顺铂先或后合用两组的脾脏指数和净体重 ,与易瑞莎组比较明显下降 (P <0 0 1)。结论 易瑞莎对小鼠H2 2肝癌移植瘤生长具有显著抑制作用。

OBJECTIVE TO INVESTIGATE THE EFFECT OF IRESSA (GEFITINIB,ZD1839) ON H22 MURINE HEPATOCELLULAR CARCINOMA.METHODS MICE BEARING H22 HEPATOCELLULAR CARCINOMA WERE RANDOMLY DIVIDED INTO ORAL CONTROL GROUP,NORMAL SALINE (NS)CONTROL GROUP,CISPLATIN(CDDP)D1-5 GROUP, CDDP D6-10 GROUP, IRESSA GROUP, IRESSA COMBINED WITH CDDP EARLY (IRESSA +CDDP D1-5)GROUP, AND IRESSA COMBINED WITH CDDP LATELY(IRESSA+CDDP D6-10) GROUP. IRESSA WAS GIVEN BY DAILY GASTROGAVAGE FOR 10 DAYS(DAY1-DAY10) AT 100MG/KG IN BODY WEIGHT(BW).CDDP WAS GIVEN BY DAILY INTRAPERITONEAL INJECTION FOR 5 DAYS(DAY1-DAY5,OR DAY6-DAY10) AT 1.2 MG/KG IN BW. THE GROWTH INHIBITING RATE(IR) OF TUMOR,CHANGE OF BW, SPLEEN INDEX (SI), AND AMOUNTS OF BLOOD LEUCOCYTE OR HEMOGLOBIN WERE DETECTED.RESULTS IR OF TUMOR IN IRESSA GROUP WAS NOT SIGNIFICANTLY DIFFERENCE WITH THAT IN CDDP D1-5 GROUP,CDDP D6-10 GROUP, IRESSA +CDDP D1-5 GROUP(P>0.05).IR OF TUMOR IN IRESSA GROUP,CDDP D1-5 GROUP, CDDP D6-10 GROUP, IRESSA AND IRESSA+CDDP D1-5 GROUP WERE 41%, 54%, 46%,AND 56%, RESPECTIVELY. IR OF TUMOR IN IRESSA +CDDP D6-10 GROUP(26%) WAS SIGNIFICANTLY LOWER THAN THAT IN CDDP D6-10 GROUP(P <0.05) OR IN IRESSA +CDDP D1-5 GROUP(P <0.01). COMPARED WITH ORAL OR NS CONTROL GROUPS, SI AND NET BW IN IRESSA GROUP WAS NOT SIGNIFICANTLY DIFFERENCE(P>0.05).SI AND NET BW IN BOTH IRESSA +CDDP D1-5 GROUP AND IRESSA +CDDP D6-10 GROUP WERE LOWER MARKEDLY THAN THOSE IN IRESSA GROUP (P <0.01). CONCLUSION TUMOR GROWTH OF H22 BEARING MICE WAS MARKEDLY INHIBITED BY IRESSA.