不同液体复苏方式对失血性休克大鼠小肠黏膜细胞凋亡的影响

Effects of different means of fluid resuscitation on apoptosis of small intestinal mucosa in rats with hemorrhagic shock.

目的 探讨不同液体复苏方式对失血性休克大鼠小肠黏膜细胞凋亡的影响。方法 32只SD大鼠 ,制成未控制性重度失血性休克模型 ,随机分成对照组、NF组 (无液体复苏组 )、NS4 0组 (限制性液体复苏组 )和NS80组 (快速大量液体复苏组 ) ,检测和比较失血 /复苏 /急救后各组存活大鼠小肠黏膜细胞凋亡的发生情况。结果 早期液体输注可显著降低大鼠的早期死亡率 ;各组大鼠中存活者的小肠黏膜细胞均有大量凋亡发生 ,其中与NF组和NS4 0组相比较 ,NS80组存活大鼠的小肠黏膜细胞凋亡显著增加 (P <0 0 1)。结论 在上述液体复苏方式中 ,限制性液体复苏可显著降低未控制性重度失血性休克大鼠的早期死亡率和小肠黏膜细胞凋亡 。

Objective To investigate the effects of different means of fluid resuscitation on apoptosis of small intestinal mucosa in rats with hemorrhagic shock.Methods A model of rat with severe hemorrhagic shock and active bleeding was established in 32 SD rats.The rats were randomly divided into control group? NF group (no fluid resuscitation group), NS40 group (controlled fluid resuscitationgroup) and NS80 group (rapid large_volume fluid resuscitation group).We detected and compared the apoptosis of small intestinal mucosa of survivors after hemorrhage and resuscitation.Results Fluid resuscitation reduced the early death rate of rats markedly.There was marked apoptosis in small intestinal mucosa of all survivors.Compared with NF group and NS40 group,the apoptosis of small intestinal mucosa of NS80 group was obviously increased (P<0.01).Conclusion Among three means of fluid resuscitation, controlled fluid resuscitation can obviously reduce the early death rate and the apoptosis of small intestinal mucosa in rats with severe hemorrhagic shock,which may be of benefit in improving prognosis.