补骨脂素逆转人乳腺癌细胞多药耐药性的研究

Reversal of multidrug resistance in human breast cancer cell by psoralen

目的 研究中药补骨脂素对人乳腺癌细胞多耐药性的逆转作用。方法 用MTT法测定药物的细胞毒性和IC50 用流式细胞仪测定耐药细胞P170糖蛋白表达 ,并选异搏定作阳性对照 ,观察具有钙拮抗作用的补骨脂素对MCF 7/ADR多药耐药性的逆转作用。结果 补骨脂素在非细胞毒性剂量下能使MCF 7/ADR对阿霉素的浓度升高 ,但对细胞表面的糖蛋白P 170却没有影响。结论 补骨脂素具有逆转人乳腺癌MCF 7/ADR多药耐药性的作用。

Objective To investigate the effect of reversing multidrug resistance (MDR) of psoralen in human breast cancer cell. Methods Psoralen, one kind of herd medicine, was selected and its reversal effect to MCF 7/ADR cell line was studied. MTT method used to determine the toxic effect and IC 50 and was used to examine the expression of P170 protein of multidrug resistance cell. Verapamil was used as positive control.Results Psoralen could reduce the value of IC 50 of adriamycin against MDR cells line MCF 7/ADR at non cytotoxic concentration and enhance the accumulation of adriamycin,but no marked change in expression of P 170 protein was observed. Conclusion Psoralen can reverse MDR of MCF 7/ADR cell of human breast cancer.