胰岛α细胞培养上清液对INS-1细胞胰岛素释放与合成的影响

Effect of α cell of islets of langerhans cultured supernatant on insulin releasing and mRNA level in INS-1 cells line

在培养的INS 1细胞中 ,分别加入不同比例的胰岛α细胞培养上清液 (以下简称上清液 ) ,在不同浓度葡萄糖的刺激下 ,分别孵育不同时间 ,用放射免疫法测定INS 1细胞培养基中的胰岛素含量。在 2 0mmol L葡萄糖浓度下 ,不同刺激时间 ,不同浓度的上清液刺激INS 1细胞分泌的胰岛素显著高于 0 %上清液 (以下简称对照组 ,P <0 0 5或0 0 1 )。在 0、1 85mmol L葡萄糖刺激时 ,不同浓度的上清液对INS 1细胞的胰岛素分泌无明显作用 ,而在 5 6、1 6 7和 5 0mmol L葡萄糖刺激时 ,不同浓度的上清液刺激INS 1细胞分泌的胰岛素显著高于对照组 (P <0 0 5或 0 0 1 )。RT PCR结果显示 ,在 1 6 7mmol L葡萄糖刺激 4、1 2和 2 4h后 ,30 %上清液对INS 1细胞胰岛素mRNA水平均无明显影响。提示胰岛α细胞培养上清液对糖刺激的INS 1细胞的胰岛素分泌有促进作用 。

Different proportion of supernatant and glucose were added to INS 1 cells in varied incubation time.Insulin in media was measured by radioimmunoassay (RIA). Incubation with different proportion supernatant significantly increased insulin release in the presence of 20mmol/L glucose at different time( P <0.05 or 0.01).No significant effect of different proportion supernatant was found in the presence of 0,1.85mmol/L glucose compared with control,but insulin releasing was significantly increased in the presence of 5.6mmol/,16.7mmol/L,50mmol/L glucose as compared with INS 1 cells cultured without supernatant( P <0.05 or 0.01). RT PCR result showed that after 4h,12h,24h incubation respectively, 30% supernatant can not increased insulin messenger RNA levels significantly at the present of 16.7mmol/L glucose compared with INS 1 cells cultured with glucose alone. Our study suggests that the supernatant can increase glucose induced insulin releasing, but have no effect on insulin gene transcription and biosynthesis.