摘要
目的:观察重组腺病毒介导Osf2/Cbfa1对间充质干细胞(MSCs)的促成骨效应。方法:分离培养兔MSCs,用重组Osf2/Cbfa1腺病毒感染MSCs,蛋白印迹检测目的基因表达,对硝基苯磷酸二钠法和放免法分别检测成骨细胞标志基因碱性磷酸酶(ALP)和骨钙素表达。结果:感染后MSCs检测到Osf2/Cbfa1表达,从第4天开始,ATP活性和骨钙素含量较单纯诱导组和对照组显著增高。结论:Osf2/Cbfa1可明显促进MSCs向成骨细胞定向分化。
AIM:To evaluate the effect of Osf2/Cbfa1 mediated by recombinant adenovirus on the differentiation of mesenchymal stem cells(MSCs) into osteoblasts. METHODS:The rabbit MSCs were isolated and cultured in vitro,then infected with Osf2/Cbfa1 recombinant adenovirus.The expression of Osf2/Cbfa1 was detected by western blot analysis.Alkaline phosphatase(ALP) activity and expression of osteocalcin production as the osteoblast markers,were measured by the 4 nitrophenylphosphate method and radioimmunoassay,respectively. RUSULTS:Western blot analysis found that the infected MSCs expressed Osf2/Cbfa1 gene.ALP activity and osteocalcin production were markedly upregulated on day 4 in the infection group compared with the control group and the single inducement group. CONCLUSION:Osf2/Cbfa1 can obviously promote directed differentiation of MSCs into osteoblasts.
出处
《中国临床康复》
CSCD
2004年第14期2655-2657,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金(30300079)
第三军医大学科研基金(XG200313)~~
