脑溢安颗粒对脑出血大鼠脑内IL-6 mRNA及蛋白表达的影响

Effects of Naoyian granule on the expression of IL-6 mRNA and protein in the experimental intracerebral hemorrhagic brain in rats

目的 :探讨脑溢安颗粒 (简称脑溢安 )对脑出血的保护作用机制。方法 :采用胶原酶注射建立大鼠脑出血模型 ,以原位杂交及Westernblotting方法检测实验性脑出血大鼠脑内白介素 6 (interleukin 6 ,IL 6 )mRNA与蛋白的表达及脑溢安的影响。结果 :脑溢安治疗组脑内IL 6mRNA表达信号在术后 4～ 7d较模型组增强 ,且IL 6蛋白表达在术后 6h～ 7d均明显高于模型组 (P <0 .0 1 )。结论 :上调IL 6蛋白的表达、维持IL 6mRNA及蛋白的高水平表达可能是脑溢安保护脑出血损伤的作用机制之一。

Objective To explore the protective mechanisms of the traditional Chinese medicine complex Naoyian granule (NYA) on intracerebral hemorrhagic (ICH). Methods ICH rat models were established by stereotaxically injecting 0.4 U type Ⅶ collagenase into the right globus pallidus (GP), the expression of IL 6mRNA and protein were investigated by in situ hybridization and Western blotting. Results In the NYA group the IL 6 mRNA level was higher than that of the model group on 4～7 d and the IL 6 protein level was remarkably higher than that of the model group on 6 h ～7 d after ICH injury ( P < 0.01 ).Conclusion Up regulating the expression of IL 6 protein,and stabilizing the increasing expression of IL 6 mRNA and protein may be the protective mechanisms of NYA.