血管紧张素转换酶基因多态性对血浆血管紧张素Ⅱ水平及心衰的影响

Effect of the polymorphism of the angiotensin-converting enzyme gene on the plasma angiotensin Ⅱ levels and the risk of chronic heart failure

目的 :探讨血管紧张素转换酶 (ACE)D/I基因多态性对中国人血浆血管紧张素Ⅱ (AngⅡ )水平的影响 ,以及是否可影响心衰的发生。方法 :用多聚酶链式反应对 1 81例受检者 (1 0 2例健康体检者、79例慢性心衰患者 )进行ACE基因分型 ,用放射免疫法测定其AngⅡ水平。结果 :在 1 81例受检者中 ,ACE基因多态性与AngⅡ水平有关 ,AngⅡ水平依次为DD型 >ID型 >II型 (P <0 .0 5 )。多元逐步回归模型分析表明 ,校正多种混淆因素后AngⅡ水平仍受ACE基因型影响。ACE基因型在心衰患者和健康对照者中的分布无明显差异。结论 :国人ACED/I基因多态性与AngⅡ水平存在相关 ,携带DD型ACE基因者AngⅡ水平较高 ;

Objective To investigate the effect of the polymorphism of angiotensin converting enzyme (ACE) gene D/I on the plasma angiotensin Ⅱ levels and the risk of chronic heart failure.Methods The genotype was determined by polymerase chain reaction (PCR) in 181 subjects (102 controls and 79 patients with chronic heart failure). The levels of plasma Ang Ⅱwere also assessed by radio immunity assay (RIA) in the above subjects.Results A marked difference in the plasma AngⅡ levels was observed among the 3 genotypes(DD,ID and II). The levels of plasma AngⅡ in DD genotype class were the highest [DD: (65.43± 26.68 ),ID: (55.49±25.90),II: (46.69±23.00); P <0.05, DD vs ID; P <0.01, DD vs II].The ACE gene I/D polymorphism showed that the levels of Ang Ⅱ were still affected by the ACE genotype after correcting confounding multifactor by linear regression analysis. There was no difference in genotypes or allele frequencies between the patients and the controls in our study. Conclusion The polymorphism of ACE gene D/I is associated with higher plasma AngⅡ levels in the Chinese. No evidence supports an association between ACE genotype and the diagnosis of chronic heart failure.