检测IgG、IgM抗体对考核丝虫病疗效的观察

Observations on the Detection of IgG and IgM Antibody for Assessment of Curative Effect in Filariasis

用马来丝虫成虫冰冻切片抗原作IEST,对海群生治疗后18个月的班氏微丝蚴血症和症状体征阳性者进行了复查,结果显示治后IgG和IgM的总GMRT,均分别低于治前IgG和IgM的总GMRT;IgG和IgM的治后转阴率分别为15.1%和80.3%,差异具有显著性(P<0.01)。表明IgG抗体水平在治后18个月虽有下降。但仍有较高的抗体阳性率,而IgM抗体已大部转阴,转阴速度较快,提示在丝虫病防治后期检测IgM抗体的转阴率,可以作为考核疗效和疫情监测的一种方法。

Immunoenzymatic Staining Technique (IEST) with frozen sections of adult Brugia malayi as antigen was used to detect antibody in bancroftian filariasis. 93 subjects with microfilaremia and physical signs were reexamined in eighteen months after diethylcarbamazine (DEC) treatment. After the treatment, the total geometric mean reciprocal titers (GMRT) of specific IgG and IgM antibodies were lower than that of before treatment,the negative rates of IgG and IgM were 15.1% and 80.3%,respectively, the difference of rates was significant (P<0.01). The results showed that IgG antibody level remain relatively high,but the IgM antibody declined quickly after treatment. It is suggested that to detect IgM antibody can be used as an assessment method of curative effect and epidemiological surveillance at the late stage of filariasis control.