肾结核免疫学诊断方法对比研究

A study on the effect of immunological diagnosis of renal tuberculosis

目的:比较三种血清学诊断方法对肾结核诊断的效果。方法:采用免疫层析法和ELISA法检测血清中特异性抗体。结果:52例肾结核、105例健康人和126例肾脏病患者血清标本中,抗LAM抗体检测阳性率分别为59.6％,0％和0％;抗38ku和细胞质膜抗原抗体检测阳性率分别为82.7％、0.9％和2.4％;抗PPD抗体阳性率分别为76.9％、8.5％和11.1％。抗LAM和抗38ku抗体联合检测,肾结核患者阳性检出率达88.5％。结论:抗LAM和抗38 ku抗体快速检测方法能够在半小时内对肾结核作出初步诊断和鉴别诊断。

Objective:To compare the effect of diagnosis for renal tuberculosis by 3 kind of serological diagnosis methods . Methods; The specific antibody in the serum were detected by immunochromatography and ELISA. Results:The positive rate of anti-LAM antibody were 59. 6% , 0% and 0% respectively in the serum of 52 patients with renal tuberculosis, 105 healthers and 126 patients with renopathy; The positive rate of anti-38ku protein and cytoplasmic membrane antibody were 82.7% ,0.9% and 2.4% respectively; The positive rate of anti-PPDantibody were 76. 9% ,8.5% and 11.1% respectively. By the united detection of anti-LAM antibody and anti-38ku antibody, the positive rate of renal tuberculosis was 88. 5% . Conclusion:The rapid test of anti-LAM and Anti-38ku antibody could be applied to the preliminary diagnosis and differentiation diagnosis of real tuberculosis.