纳米级全氟碳化合物抗出血性休克和神经保护作用的实验研究

The experimental study on the function of anti-hemorrhagic shock and neuroprotection of nanometer perfluorocarbon

目的:通过建立新西兰兔出血性休克模型,观察评价纳米级全氟碳化合物抗出血性休克作用,及对脑皮质神经元的保护作用。方法:建立新西兰兔出血性休克模型,观察氟碳治疗前、后及各组各时间点的呼吸、血压、心率、血气等各参数及脑皮层病理变化。结果:12 h存活率实验组比对照组高,呼吸较对照组深大,次数加快,在监测结束时血压较对照组为高,动脉血氧分压较对照组明显升高,CO2排泄较对照组增强,对照组血气呈现明显变酸趋势。实验组瞳孔较对照组偏小,对光反射灵敏。组织学观察可见实验组脑皮层损伤较对照组明显减轻。结论:纳米级全氟碳化合物具有抗出血性休克作用,且通过多方面的作用机制对脑皮质神经元具有明显保护功能。证明氟碳是一种有效的神经保护药,在临床应用前景中,可用于缺血损害及脑血管病术中神经保护。

Objective: To establish New Zealand rabbits model to observe and view the function of effectiveness of nanometer perfluorocarbon ( PFC) in the course of treatment to hemorrhagic shock, and protection cerebral cortex nerve cells. Methods:Hemorrhagic shock was induced in New Zealand rabbits by artery bleeding. In the course of experiment, heart beat , breath rate, blood pressure, artery vigor, pupil light reflect, survival rate in 12 hours,the pathological changes of cerebral cortex were observed. Results:In 12 hours survival rate in experimental group was higher, breath was deeper and breath rate was also faster,blood pressure and PaO2 was significantly higher than that in control group,PaC02 evacuated higher than that. The control group had the tendency of going acid. Pupil light reflect in experimental group was more sensitive than that in control group. Pathological observing showed the parietal lobe cortex in experimental group was damaged less than that in control group. Conclusion:The nanometer PFC possess the function of anti-hemorrhagic shock, the merit of clear protection cerebral cortex neuron by various ways, and can be used as neuroprotective agents in the clinician practice of brain ischemia and operation on brain blood vessel diseases.