家兔心肌缺血再灌注后肺中bcl-2、bax表达的改变

Changes of the expression of bcl-2,bax in the lungs of rabbits after myocardial ischemia reperfusion

目的 研究家兔心肌缺血再灌注后肺中bcl 2、bax的表达 ,探讨心肌缺血再灌注后对肺组织的损伤。方法 建立心肌缺血再灌注模型 (缺血 30min ,再灌注 6 0min) ,于再灌注结束后检测血清中SOD、MDA含量 ,用免疫组织化学SABC法观察肺中bcl 2和bax的表达情况。结果 家兔心肌缺血再灌注后血清中SOD浓度减少 ,MDA浓度升高。bcl 2、bax免疫阳性细胞主要位于肺组织中血管内皮细胞和肺泡上皮细胞 ,正常时两者微量表达。再灌注 6 0min后两者光密度值均明显上升 ,但两组bcl 2光密度值没有统计学差别 ,而bax光密度值差别显著 (P <0 .0 5 )。结论 家兔心肌缺血再灌注后引起bcl 2、bax阳性细胞在肺组织中的表达变化并可能引起肺组织细胞凋亡和损伤。

Objective To investigate the changes of the expression of bcl-2、bax in the lungs of rabbits ,and to find out injuries to the lungs by myocardial ischemia reperfusion . Methods The models of myocardial ischemia reperfusion (ischemia 30min,reperfusion 60min) were made,the concentration of SOD、MDA in the blood serum was detected,and the expressions of bcl-2 and bax in the lungs were observed by the immunohistochemical SABC method after the reperfusion. Results After myocardial ischemia reperfusion ,the concentration of SOD decreased,while the concentration of MDA increased.The positive cells of bcl-2 and bax were observed in vascular endothelial cells and alveolar epithelium cells of the lungs.Both of the two expessions were not obvious under normal conditions but began to increase dramatically after 60min of reperfusion. There was no significant difference between the two groups in the value of optical density(OD),howerer,the OD value of bax of ischemia reperfusion group was higher than that of sham group (P<0.05). Conclusion The changes of the expression of positive cells of bcl-2 and bax may play an important role in apotosis and cause lung injury after myocardial ischemia reperfusion .