大鼠胰肠重建式单纯胰腺移植动物模型的构建

Experimental study on model of pancreas transplantation alone with enteric drainage in rats

目的 :研究建立胰肠重建式大鼠单纯胰腺移植(pancreastransplantationalone,PTA)的方法 .方法 :SD大鼠分为 :对照组 (NDC组 ,n =1 0 )和PTA组 (n =2 2 ) ;糖尿病大鼠采用脲链霉素诱导 ,PTA组血管吻合采用供胰腺腹主动脉绊 (含腹腔动脉及脾动脉 )及门静脉绊 (含脾静脉 )与受体腹主动脉及左肾静脉分别行端侧和端端吻合 (袖套式 ) ;胰腺断端与小肠行Roux y式吻合 .并于术前和术后 1 ,3,7,1 4和 30d监测受体质量、饮水量、进食量、尿量和空腹血糖 .结果 :PTA组平均手术时间 :供体为 (32 .2± 1 2 .7)min ,受体为 (6 3.4± 1 5 .9)min ,移植物均无热缺血时间 ,冷缺血时间为 (4 8.6± 1 8.3)min .PTA组除 1 1例 (5 0 % )于 1mo内死亡或胰腺失去功能外 ,均成活 1mo以上 ;并发症主要为胰漏及继发性胰腺炎 (7例 ,31 .8% ) ;成功的胰腺移植后 1d血糖即下降 ,3d达到正常 ;饮水量、进食量、尿量均减少 ,1 4d后基本稳定 .结论

AIM: To establish the rat model of pancreas transplantation alone (PTA) with enteric drainage. METHODS: Inbred SD rats were randomly assigned into 2 groups: a group of 10 nondiabetic rats as control and a group of 22 diabetic rats, which received PTA from the normal donors. Diabetic rats were induced by streptozotocin. Venous drainage of PTA was performed using end to side anastomosis between the donors abdominal aorta and recipients abdominal aorta segment, and end to end anastomosis between the donors portal vein segment and recipients left renal vein (with a cuff). Pancreas exocrine drainage was made by pancreas intestine anastomosis (Roux Y). The blood glucose, body weight, food intake, water intake and urine volume were monitored before transplantation and on the 1st, 3rd, 7th, 14th and 30th day after transplantation. RESULTS: The mean duration of operation of PTA group in donors was (32.2±12.7) min, and that in recipients was (63.4±15.9) min, and the mean duration of cold ischemic time was 0 min and that of warm ischemic time was (48.6±18.3) min. 11 (50%) of the 22 cases in PTA group died or lost the endocrine function within 1 month. The main complications were pancreastitis and pancreas leak after transplantation (7 cases, 31.8%). The blood glucose of all successful recipients lowered on the first day after transplantation and restored to normal on the third day. The food intake, water intake and urine volume decreased and became stable after 14 days. CONCLUSION: Successful PTAs can restore the pancreatic endocrine function of the diabetic rats.