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免疫共沉淀探讨septin蛋白家族间的相互作用 被引量：1

Co-immunoprecipitation for interactions of three human septin proteins
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摘要 目的 :探讨人肝癌中表达的 3种septin蛋白 1 ,2和6自身之间和相互之间是否存在相互作用关系 ,从而探讨在肝癌中septin家族的作用方式 .方法 :通过构建septin家族基因的pCMV Myc ,pCMV HA表达载体 ,采用人肾 2 93细胞内蛋白的免疫共沉淀进行验证蛋白的相互作用 .结果 :Septin家族蛋白中确实存在着相互作用 ,不仅蛋白相互之间存在相互作用 ,且同一种蛋白质的分子之间也存在着较强的相互作用 .结论 AIM: To study the interactions of three human septin proteins, septin 1, 2 and 6, and to investigate the working model of the septins in the hepatic carcinoma. METHODS: Full length septin 1, 2 and 6 gene were cloned into the pCMV Myc and pCMV HA vector respectively and co immunoprecipitation experiment in human 293 was used to investigate the interactions of the three septin proteins. RESULTS: Interactions in the septin family were confirmed, including the interactions between septin 1 and septin 2 or septin 6, and the interactions within themselves. CONCLUSION: Septin proteins as a complex may play an important role in the carcinogenesis by regulating cell cycles.
作者 周献锋 曹建平 彭佶松 罗家林 朱巍 郑斯英 朱财英
机构地区 苏州大学放射医学与公共卫生学院放射生物学教研室 Department of Human Genetics
出处 《第四军医大学学报》 北大核心 2004年第10期898-900,共3页 Journal of the Fourth Military Medical University
关键词 septin蛋白家族 免疫共沉淀 细胞周期蛋白质 肝癌 单克隆抗体 septin protein family co-immunoprecipitate cell cycle proteins
分类号 R735.7 [医药卫生—肿瘤]
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参考文献8

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同被引文献14

  • 1Huang Chaochun, Zhou Jialiang, Wu Shiliang, et al. Cloning and tissue distributrion of a the human β3GalT7 gene, a member of the β1,3-Glycosyltransferase family[J]. Glyconjugate, 2004,21 (5):265-271.
  • 2Hiroyasu lshida, Akira Togayaehi, Tokiko Sakai, et al, A novel β1,3-N-acetylglucosaminyltransferase (β3Gn-TS), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer[J]. FEBS Letters, 2005,579 ( 1 ):71-78.
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  • 6Dong C, Temann UA, Flavell RA. Cutting edge:critical role of inducible Costimuhtor in germinal center reactins [J]. Immunol, 2001366(6):3659-3622.
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  • 8Valerie Marin, Gilles Kaplanski, Sandra Gres, et al. Endothelial cell culture:protocol to obtain and cultivate human umbilical endothelial cells[J]. Immunol Methods, 2001,254( 1-2):183-190.
  • 9Zhou Ying-Hui, Chen Yong-Jing, Ma Zhen-Yu, et oi. 4IgB7-H3 is the major isoform expressed on immunocytes as well as malignant cells [J]. Tissue Antigens, 2007,70(2):96-104.
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第四军医大学学报
2004年 第10期

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