核因子κB,Bcl-2在肝癌的表达及其相关性

Expression of NF-κB in human hepatocellular carcinoma and its relation to Bcl-2 expression

目的 :探索核因子κB(NF κB)在肝癌的表达变化 ,以及与病理分期和抗凋亡基因Bcl 2表达的相关性 .方法 :采用免疫组织化学方法 ,检测NF κB在 4 0例肝细胞肝癌及 1 5例正常肝组织中的表达变化及抗凋亡基因Bcl 2的相应表达 .结果 :NF κB在肝癌组和正常肝组织组的阳性表达率分别为72 %、1 3% ,两者有显著性差异 (P <0 .0 1 ) ,而强阳性表达率分别 30 %和 7% ,Bcl 2在肝癌组和正常肝组织组的阳性表达率分别为 4 0 %和 7% ,两者有显著性差异 (P <0 .0 5 ) ,而强阳性表达率分别为 1 7%和 0 .NF κB的阳性表达在病理分级间有显著性差异 (P <0 .0 1 )且与Bcl 2的阳性表达有显著相关(r =0 .389,P =0 .0 1 3) .结论 :NF κB异常表达与肝癌的发生有关 ,并在一定程度上可以解释肝癌对化疗的抵抗作用 .NF κB与Bcl 2高度相关 ,表明NF κB可以通过对下游抗凋亡基因表达的调控 。

AIM: To explore NF κB expression in liver carcinoma tissues and its correlation with the pathological stage of cancer tissues and the expression of Bcl 2. METHODS: By means of immunohistochemical SABC method, the expressions of NF κB and Bcl 2 were compared between the hepatocellular carcinoma tissues from 40 cases and normal liver tissues from 15 cases. RESULTS: The positive expression and the strong positive expression of NF κB in the liver cancer group and the control group were 72%, 13% ( P <0.01) and 30%, 7% ( P <0.05) respectively, both with significant difference. The positive expression and strong positive expression of Bcl 2 were 40%, 7% ( P <0.05) and 17%, 0 respectively. The positive expression of NF κB was significantly different for different pathological grades ( P <0.01) and the expression of Bcl 2 was closely related to NF κB ( r=0.389, P =0.013). CONCLUSION: The abnormal expression of NF κB is closely associated with the development of liver cancer and the expression of NF κB is significantly different for different pathological grades. NF κB is closely related to Bcl 2, which indicates that NF κB is involved in the anti apoptosis of liver cancer cell by mediating anti apoptosis gene.