中药肉苁蓉含药血清对PC12细胞凋亡的影响

Effects of Cistanche on apoptosis of PC12 cells

目的 :观察中药肉苁蓉含药血清对PC1 2细胞凋亡的影响 .方法 :采用 1 甲基 4 苯基吡啶离子 (1 methyl 4 phenylpyridinium ,MPP+ )建立PC1 2细胞凋亡模型 ,应用血清药理学方法 ,结合光镜荧光染色核形分析、透射电镜、流式细胞仪等检测 ,研究中药兔血清对PC1 2 ,细胞凋亡的保护作用 .结果 :含 1 /2和 2倍等效剂量组中药兔血清 (ig剂量为肉苁蓉生药 1 .6 ,3.2和 6 .4 g/kg)处理的PC1 2细胞经MPP+ 作用后 ,凋亡率分别为 1 6 .0 % ,1 0 .2 %和 2 .8% ,与空白组 (1 8.6 % )相比差异显著 (P <0 .0 5 ) .结论 :肉苁蓉具有拮抗MPP+

AIM: To investigate the effects of Cistanche on 1 methyl 4 phenylpyridinium (MPP+) induced PC12 cells apoptosis. METHODS: PC12 cells were exposed to 200μmol/L MPP+ alone or MPP+ combined with different concentration of Cistanche from New Zealand White rabbit serum. Effects of Cistanche on apoptosis of PC12 Cells were detected by flow cytometry, electron microscopy and fluorescence microscopy. RESULTS: The sera collected from rabbits fed on an equivalent dosage of Cistanche or on a twofold equivalent dosage of Cistanche had obvious protective effects on PC12 cells and the apoptosis rates of PC12 cells incubated with these two sera were 2.8% and 10.2%, respectively, which were obviously lower than those of PC12 cells with MPP+ treatment only (18.6%, P <0.05). CONCLUSION: Cistanche can protect PC12 cells from apoptosis induced by MPP+.