氮酮对人参皂苷Rg_1和米诺地尔透皮吸收的影响

Effects of azone on transdermal absorption of ginsenoside Rg_1 and minoxidil

目的 :探讨氮酮作为人参米诺地尔复方制剂促渗剂的适当浓度 .方法 :应用透皮扩散装置 ,采用离体小鼠背部皮肤作为屏障 ,以生理盐水为接受介质 ,研究不同浓度的氮酮对人参米诺地尔复方制剂透皮吸收的影响 ,利用反相高效液相色谱法测定人参米诺地尔复方制剂的累积透皮量 .结果 :人参米诺地尔复方制剂 2 4h累积透皮量分别为 :人参皂苷Rg1 0 ,1 0 ,30 ,5 0 ,70 ,90mL/L氮酮组为 (0 .2 6± 0 .0 2 ) ,(3.4 7±0 .1 6 ) ,(3.98± 0 .2 3) ,(4 .5 4± 0 .73) ,(2 .36±0 .1 1 ) ,(1 .6 5± 0 .0 8)mg/L ;米诺地尔 0 ,1 0 ,30 ,5 0 ,70 ,90mL/L氮酮组为 (8.87± 0 .1 8) ,(1 1 .2 1± 0 .2 0 ) ,(1 2 .73±0 .36 ) ,(1 1 .72± 0 .36 ) ,(7.2 2± 1 .2 9) ,(4 .6 5± 0 .2 6 )mg/L .结论 :在离体小鼠皮肤 ,氮酮作为促渗剂的最佳浓度为 30mL/L .

AIM: To study the proper mass concentration of azone used as the permeator of ginseng minoxidil preparation. METHODS: Using a device of transdermal diffusion and using the mouse skin in vitro as barrier and 9 mg/L NaCL as accepted medium, we studied the effects of azone with different concentrations on transdermal absorption. RP HPLC method was adopted to measure the amount of transdermal absorption of ginseng minoxidil preparation. RESULTS: The amount of transdermal absorption of ginsenoside Rg 1 within 24 h was (0.26±0.02), (3.47± 0.16), (3.98±0.23), (4.54±0.73), (2.36±0.11) and (1.65±0.08) μg/mL in 0, 10, 30, 50, 70 and 90 mL/L azone groups, respectively. The amount of transdermal absorption of minoxidil within 24 h was (8.87±0.18), (11.21± 0.20), (12.73±0.36), (11.72±0.36), (7.22±1.29) and (4.65±0.26) μg/mL in 0, 10, 30, 50, 70 and 90 mL/L azone groups, respectively. CONCLUSION: 30 mL/L is the optimal concentration for azone used as a transdermal enhancer in percutaneous absorption of ginseng and minoxidil through mice skin in vitro .