葛根对地塞米松诱导的胰岛素抵抗的影响

Influence of Puereria thomsonii on insulin resistance induced by dexamethasone

目的 :探讨葛根提取物对地塞米松造成的胰岛素抵抗的影响及其作用机理。方法 :采用地塞米松诱导 3T3 L1脂肪细胞胰岛素抵抗 ,检测药物对细胞培养基中葡萄糖浓度的影响 ;采用肌肉注射地塞米松 ( 1mg·kg-1,隔日 1次 )的方法建立胰岛素抵抗动物模型 ,测定空腹血糖 (FBG)、血清胰岛素 (FINS) ,并计算胰岛素抵抗指数 (IRI)与胰岛素敏感指数 (ISI)。结果 :葛根提取物能够明显降低胰岛素抵抗 3T3 L1脂肪细胞培养基中的葡萄糖水平 ,增强细胞对胰岛素的敏感性 ;葛根提取物显著降低胰岛素抵抗大鼠空腹血清胰岛素水平及胰岛素抵抗指数 ,有效改善了大鼠的胰岛素抵抗状态。结论

Objective:To investigate the influence of Puereria thomsonii on insulin resistance induced by dexamethasone. Method:3T3 L1 adipocytes model of insulin resistance was made by dexamethasone and the change of glucose concentration in cell culture was determined after action of drugs. Rat animal model of insulin resistance was made by intramuscular dexamethasone (1 mg·kg -1 , every other day), and fasting blood glucose(FBG) and fasting serum insulin (FINS) were enamined, and at the end of the experiment, insulin sensitive index(ISI) and insulin resistance index(IRI) were calculated. Result: P. thomsonii decreased the concentration of glucose in 3T3 L1 adipocytes culture significantly and improved the sensitivity of 3T3 L1 adipocytes to insulin. P. thomsonii improved the sensitivity of rats to insulin and diminished the fasting serum insulin and insulin resistance index. Conclusion: P. thomsonii can significantly improve insulin resistance induced by dexamethasone.