左旋四氢巴马汀对脑缺血小鼠脑组织氨基酸类神经递质含量的影响

Effects of L-tetrahydropalmatine on concentrations of neurotransmitter amino acids in mice with cerebral ischemia

目的 :通过观察左旋四氢巴马汀对脑缺血小鼠脑组织氨基酸类神经递质含量的影响 ,探讨左旋四氢巴马汀抗脑缺血作用的可能机制。方法 :动物分为对照组 ,模型组 ,左旋四氢巴马汀低、中、高剂量组 ,银杏组 ,采用脑匀浆法结合HPLC法分别测定各组小鼠脑组织中Glu ,GABA含量。结果 :模型组小鼠脑组织Glu和GABA含量均较对照组明显上升 ,Glu/GABA明显升高。左旋四氢巴马汀能明显降低脑缺血小鼠脑组织Glu含量 ,使Glu/GABA趋于正常。结论 :左旋四氢巴马汀可能通过降低脑缺血大鼠脑组织Glu含量 。

Objetive:To observe the effects of L tetrahydropalmatine( L THP)on concentrations of neurotransmitter amino acids in mice with cerebral ischemia and to discuss the mechanisms of L THP in tyreating cerebral ischemia. Method:Mice were randomly divided into six groups:control group, cerebral ischemia group, L THP treated group(doses were 14, 28, 56 mg·kg -1 respectively)and Ginkgo biloba extract treated group. Concentrations of Glu and GABA were determined by HPLC. Result:Compared with control group, Glu and GABA in mice with cerebral ischemia were much higher. The ratio of Glu/GABA increased significantly. L THP markedly reduced the concertrations of Glu and the ratio of Glu/GABA in mice with cerebral ischemia. Conclusion:Decrease in Glu concertration and ratio of Glu/GABA may be one of the mechanisms of L THP in treating cerebral ischemia.