抗胃腺癌免疫核糖核酸有效成分构象分析

CONFORMATIONAL ANALYSIS OF EFFECTIVE COMPONENTS OF ANTI-GASTRIC CANCER IRNA

本研究将NRNA、ATIRNA和ATIRNAⅠ～Ⅴ组份进行了抑癌活性对比分析,证实以ATIRNAⅠ、Ⅱ组份活性最强。继而用园二色谱仪对ATIRNAⅠ、Ⅱ组份进行了CD谱分析,参照SRNA、NRNA、ATIRNA和ATIRNAⅢ、Ⅳ、Ⅴ组份的CD谱分析。结果发现,ATIRNAⅠ、ⅡCD谱明显不同于其它各种RNA的CD谱;ATIRNAⅠ、Ⅱ的构象在低盐浓度条件下,兼有A、Z和其它型构象,具有较大分子柔顺性;在高盐浓度条件下会发生Z→A型构象转变;而其它各种RNA只呈单纯A型构象,分子刚性较强,在高盐浓度条件下有A→Z型构象转变趋势。

It was verified that the components Ⅰ and Ⅱ of ATIRNA posses stronger inhibiting effect on cancer by analysis of inhibiting cancer activity for components Ⅰ to VVV of ATIRNA and NRNA as well as ATIRNA. CD spectrum of components Ⅰ , Ⅰ of ATIRNA was analyzed by Jasco. It was found that CD spectrum of ATIRNA Ⅰ. Ⅱ was different from that of other RNA. At low salt concentration, the conformation of ATIRNA Ⅰ ,Ⅱ showed A, Z and other forms, with more flexibility. At high salt concentration, it undergoes a transition of conformation from Z to A, with less flexibility, as well as a tendency of transition from A to Z.